CYAO: Cyanobacterial Platform Optimised for Bioproduction
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Media: BG11 TAP BBM F/2
BBM Recipe Stock 1A, Nitrate 10 mL Stock 1B, Calcium 10 mL Stock 1C, Magnesium 10 mL Stock 1D, Phosphate DB 10 mL Stock 1E, Phosphate MB 10 mL Stock 1F, Sodium 10 mL Stock 2, Alkaline EDTA 1.0 mL Stock 3, Acidified Iron 1.0 mL Stock 4, Boron 1.0 mL Stock 5, Micro-elements 1.0 mL Adjust pH between 6.6 and 6.8 To 1 L Distilled H20
Stocks Major Salt Stocks Solutions 1A-1F: 1A NaNO3 10 g 1B CaCl2 2H2O 1 g 1C MgSO4 7H2O 3 g 1D K2HPO4 3 g 1E KH2PO4 7 g 1F NaCl 1 g Each to 400 mL Distilled H20 Stoc 2, Alkaline EDTA: EDTA anhydrous 50 g KOH 31 g To 1 L Distilled H20 Stock 3, Acidified Iron: FeSO4 7H2O 4.98 g H2SO4 1.0 mL To 1 L Distilled H20 Stock 4, Boron: H3BO3 11.42 mL To 1 L Distilled H20 Stock 5, Micro-Elements: ZnSO4 7H2O 8.82 g MnCl2 4H2O 1.44 g MoO3 0.71 g CuSO4 5H2O 1.57 g Co(NO3)2 6H20 0.49 g To 1 L Distilled H20 Agar Plates Add 1.5-2% w/v of Noble Agar to medium (typical 6-8 g to 400 mL of BBM solution) BBM receipt in PDF format: bbm.pdf
The research is founded by the Cariplo Foundation under the Biotechnology founding scheme.
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