CYAO: Cyanobacterial Platform Optimised for Bioproduction
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Media: BG11 TAP BBM F/2
BG11 Recipe Iron/Citrate Stock 1 10 mL Magnesium Stock 2 10 mL Phosphate Stock 3 10 mL Microelements Stock 5 1.0 mL NaNO3 1.5 g Na2CO3 0.02 g To 1 L Distilled H20 Adjust pH to 7.5 with HCl
Stocks Iron/Citrate Stock 1: Na2Mg EDTA 0.1 g Ferric ammonium citrate 0.6 g Citric acid H2O 0.6 g CaCl2 2H2O 3.6 g To 1 L Distilled H20 Magnesium Stock 2: MgSO4 H2O 7.5 g to 1 L Distilled H20 Phosphate Stock 3: K2HPO4 3H2O 4.0 g To 1 L Distilled H20 Micro-Elements Stock 5: H3BO3 2.86 g MnCl2 4H2O 1.81 g ZnSO4 7H2O 0.222 g CuSO4 5H2O 0.079 g CoCl2 6H2O 0.050 g NaMoO4 2H2O 0.391 g to 1 L Distilled H20 Agar Plates - Prepared 2x BG 11 (200 or 400 mL) + 10 mM TES pH 8.2. - Prepare 3% w/v Agar in milliQ H20 + 1 mg Na-Thiosulphate (200 mL or 400 mL) - Autoclave both solution - Let them cool to about 50 °C, mix equal volumes, then pour plates BG11 recipeit in PDF format: BG11.pdf
The research is founded by the Cariplo Foundation under the Biotechnology founding scheme.
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